Efficient synthesis and replication of diverse sequence libraries composed of biostable nucleic acid analogues
John R. D. Hervey, Niklas Freund, Gillian Houlihan, Gurpreet Dhaliwal, Philipp Holliger, Alexander I. Taylor
Abstract
Blends of engineered polymerases enable efficient DNA-templated synthesis and reverse transcription of diverse-sequence oligonucleotide libraries composed of locked nucleic acid (LNA), 2′- O -methyl-RNA (2′OMe-RNA), or mixtures of the two.
Topics & Concepts
Nucleic acidSequence (biology)Replication (statistics)Computational biologyChemistryBiologyCombinatorial chemistryGeneticsVirologyAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanismsCRISPR and Genetic Engineering