Proteins in Tumor-Derived Plasma Extracellular Vesicles Indicate Tumor Origin
Meltem Barlin, Petra Erdmann-Gilmore, Jacqueline L. Mudd, Qiang Zhang, R. W. Seymour, Zhanfang Guo, Julia R. Miessner, S. Peter Goedegebuure, Ye Bi, Omar A. Osorio, Jennifer Alexander‐Brett, Shunqiang Li, X. Cynthia, Ryan C. Fields, R. Reid Townsend, Jason M. Held
Abstract
Cancer-derived extracellular vesicles (EVs) promote tumorigenesis, premetastatic niche formation, and metastasis via their protein cargo. However, the proteins packaged by patient tumors into EVs cannot be determined in vivo because of the presence of EVs derived from other tissues. We therefore developed a cross-species proteomic method to quantify the human tumor-derived proteome of plasma EVs produced by patient-derived xenografts of four cancer types. Proteomic profiling revealed individualized packaging of novel protein cargo, and machine learning accurately classified the type of the underlying tumor.