ID23-2: an automated and high-performance microfocus beamline for macromolecular crystallography at the ESRF
Max Nanao, Shibom Basu, U. Zander, Thierry Giraud, John Surr, Matias Guijarro, Mario Lentini, Franck Felisaz, Jérémy Sinoir, Christian Morawe, Amparo Vivo, Antonia Beteva, Marcus Oscarsson, Hugo Caserotto, Fabien Dobias, David Flot, Didier Nurizzo, Jonathan Gigmes, Nicolas Foos, R. Siebrecht, Thomas Roth, Pascal Théveneau, Olof Svensson, Gergely Papp, Bernard Lavault, F. Cipriani, R. Barrett, C. Clavel, Gordon A. Leonard
Abstract
ID23-2 is a fixed-energy (14.2 keV) microfocus beamline at the European Synchrotron Radiation Facility (ESRF) dedicated to macromolecular crystallography. The optics and sample environment have recently been redesigned and rebuilt to take full advantage of the upgrade of the ESRF to the fourth generation Extremely Brilliant Source (ESRF-EBS). The upgraded beamline now makes use of two sets of compound refractive lenses and multilayer mirrors to obtain a highly intense (>10 13 photons s −1 ) focused microbeam (minimum size 1.5 µm × 3 µm full width at half-maximum). The sample environment now includes a FLEX-HCD sample changer/storage system, as well as a state-of-the-art MD3Up high-precision multi-axis diffractometer. Automatic data reduction and analysis are also provided for more advanced protocols such as synchrotron serial crystallographic experiments.