Genome-Wide Essentiality Analysis of <i>Mycobacterium abscessus</i> by Saturated Transposon Mutagenesis and Deep Sequencing
Dalin Rifat, Liang Chen, Barry N. Kreiswirth, Eric L. Nuermberger
Abstract
Limited knowledge regarding Mycobacterium abscessus pathogenesis and intrinsic resistance to most classes of antibiotics is a major obstacle to developing more effective strategies to prevent and mitigate disease. Using optimized procedures for Himar1 transposon mutagenesis and deep sequencing, we performed a comprehensive analysis to identify M. abscessus genetic elements essential for in vitro growth and compare them to similar data sets for M. tuberculosis and M. avium subsp. hominissuis .
Topics & Concepts
Mycobacterium abscessusBiologyTransposon mutagenesisTransposable elementGeneGeneticsMycobacterium tuberculosisGenomeMycobacteriumTuberculosisBacteriaMedicinePathologyMycobacterium research and diagnosisTuberculosis Research and EpidemiologyInfectious Diseases and Mycology