Pushing the Boundaries of Resolution in Solid-State Nuclear Magnetic Resonance of Biomolecules with 160 kHz Magic-Angle Spinning
Z. Sun, Claire Ollier, Adrienn Rancz, Batiste Thienpont, Kristof Grohe, Lukas Becker, Armin Purea, Frank Engelke, Sebastian Wegner, James N. Sturgis, Tatyana Polenova, Tanguy Le Marchand, Guido Pintacuda
Abstract
We present ultrafast magic-angle spinning (MAS) at 160 kHz in solid-state nuclear magnetic resonance (NMR), demonstrating unprecedented spectral quality and coherence lifetimes in proton-detected experiments on biomolecular systems. By optimizing experimental and sample conditions and utilizing advanced filling and handling tools, we achieve superior resolution on both microcrystalline and membrane-reconstituted proteins, paving the way for new applications in structural biology.
Topics & Concepts
ChemistryMagic angle spinningSolid-state nuclear magnetic resonanceMicrocrystallineSpinningBiomoleculeUltrashort pulseMagic angleNuclear magnetic resonanceCoherence (philosophical gambling strategy)Solid-stateNuclear magnetic resonance spectroscopyOpticsPhysicsCrystallographyPhysical chemistryOrganic chemistryPolymer chemistryLaserBiochemistryQuantum mechanicsAdvanced NMR Techniques and ApplicationsProtein Structure and DynamicsNMR spectroscopy and applications