Gold nanocluster-based fluorescent sensors for<i>in vitro</i>and<i>in vivo</i>ratiometric imaging of biomolecules
S. Santhoshkumar, Manivannan Madhu, Wei‐Bin Tseng, Wei‐Lung Tseng
Abstract
bioimaging of target analytes. Additionally, the review delves into the sensing mechanisms of AuNC-based ratiometric probes, their synthetic strategies, and the challenges encountered when using AuNCs for ratiometric bioimaging. Moreover, we explore the application of protein-stabilized AuNCs and thiolate-capped AuNC-based ratiometric fluorescent probes for biosensing and bioimaging. Two primary methods for assembling AuNCs and fluorophores into ratiometric fluorescent probes are discussed: triggered assembly and self-assembly. Finally, we address the challenges and issues associated with ratiometric bioimaging using AuNCs and propose future directions for further advancing AuNCs as ratiometric imaging agents.