Comparative Effects of Domain-Specific Human Monoclonal Antibodies Against LGI1 on Neuronal Excitability
Josefine Sell, Vahid Rahmati, Marin Kempfer, Sarosh R. Irani, Andreas Ritzau‐Jost, Stefan Hallermann, Christian Geis
Abstract
<h3>Background and Objectives</h3> Autoantibodies to leucine-rich glioma inactivated protein 1 (LGI1) cause an autoimmune limbic encephalitis with frequent focal seizures and anterograde memory dysfunction. LGI1 is a neuronal secreted linker protein with 2 functional domains: the leucine-rich repeat (LRR) and epitempin (EPTP) regions. LGI1 autoantibodies are known to interfere with presynaptic function and neuronal excitability; however, their epitope-specific mechanisms are incompletely understood. <h3>Methods</h3> We used patient-derived monoclonal autoantibodies (mAbs), which target either LRR or EPTP domains of LGI1 to investigate long-term antibody-induced alteration of neuronal function. LRR- and EPTP-specific effects were evaluated by patch-clamp recordings in cultured hippocampal neurons and compared with biophysical neuron modeling. K<sub>v</sub>1.1 channel clustering at the axon initial segment (AIS) was quantified by immunocytochemistry and structured illumination microscopy techniques. <h3>Results</h3> Both EPTP and LRR domain-specific mAbs decreased the latency of first somatic action potential firing. However, only the LRR-specific mAbs increased the number of action potential firing together with enhanced initial instantaneous frequency and promoted spike-frequency adaptation, which were less pronounced after the EPTP mAb. This also led to an effective reduction in the slope of ramp-like depolarization in the subthreshold response, suggesting K<sub>v</sub>1 channel dysfunction. A biophysical model of a hippocampal neuron corroborated experimental results and suggests that an isolated reduction of the conductance of K<sub>v</sub>1-mediated K<sup>+</sup> currents largely accounts for the antibody-induced alterations in the initial firing phase and spike-frequency adaptation. Furthermore, K<sub>v</sub>1.1 channel density was spatially redistributed from the distal toward the proximal site of AIS under LRR mAb treatment and, to a lesser extant, under EPTP mAb. <h3>Discussion</h3> These findings indicate an epitope-specific pathophysiology of LGI1 autoantibodies. The pronounced neuronal hyperexcitability and SFA together with dropped slope of ramp-like depolarization after LRR-targeted interference suggest disruption of LGI1-dependent clustering of K<sup>+</sup> channel complexes. Moreover, considering the effective triggering of action potentials at the distal AIS, the altered spatial distribution of K<sub>v</sub>1.1 channel density may contribute to these effects through impairing neuronal control of action potential initiation and synaptic integration.