Engineering Perovskite Hydroxide as a Cold-Adapted Oxidase Mimic for Construction of the Robust Low-Temperature Adaptive Biosensors
Yufeng Liu, Jing Zhang, Yuxin Wan, Cong Li, Shuai Cui, Xuejiao J. Gao, Hui Wei, Dongzhi Yang
Abstract
Traditional biological detection methods rely on signal amplification strategies such as enzymatic catalysis or nucleic acid amplification. However, their efficiency decreases in low-temperature environments, compromising their detection sensitivity. To break the loss of enzyme catalytic activity at low temperatures, research on cold-adaptive nanozymes has attracted much attention. Till now, only a few nanozymes have been reported to have cold-resistant catalytic properties. Here, a new type of cold-adapted nanozyme was constructed by engineering a perovskite hydroxide. The nanozyme not only boosted the oxidase-like catalytic activity by 2 orders of magnitude but also retained excellent catalytic performance at 0 °C. This enhanced activity may be attributed to the increase in manganese content, vacancy oxygen, and tetravalent manganese. Then, a robust low-temperature adaptive biosensor was established with a cold adaptive nanozyme. Notably, the detection of sulfide ion, ascorbic acid, alkaline phosphatase, and cellular glutathione by the cold-adapted probe was less affected by the temperature reduction, and the detection sensitivity of the probe for ALP at 0 °C is better than that of a commercial kit. Finally, the cold-adapted nanozyme was further used to construct a paper-based H 2 S gas colorimetric probe. This study develops a new cold adaptive nanozyme and broadens the application scenarios of the nanozymes.