Structure-guided reshaping of the acyl binding pocket of ‘TesA thioesterase enhances octanoic acid production in E. coli
Xi Deng, Liuqing Chen, Mohan Hei, Tiangang Liu, Yan Feng, Guangyu Yang
Topics & Concepts
ThioesteraseSaturated mutagenesisBiochemistrySubstrate (aquarium)FermentationChemistryEscherichia coliEnzymeFatty acidSelectivityHydrolysisStereochemistryBiologyBiosynthesisGeneMutantCatalysisEcologyMicrobial Metabolic Engineering and BioproductionBiofuel production and bioconversionEnzyme Catalysis and Immobilization