Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing
Matthias Schmidt, Allison N. Pearson, Matthew R. Incha, Mitchell G. Thompson, Edward E. K. Baidoo, Ramu Kakumanu, Aindrila Mukhopadhyay, Patrick M. Shih, Adam M. Deutschbauer, Lars M. Blank, Jay D. Keasling
Abstract
Understanding the genetic basis of P. putida's diverse metabolism is imperative for us to reach its full potential as a host for metabolic engineering. Many target molecules of the bioeconomy and their precursors contain nitrogen. This study provides functional evidence linking hundreds of genes to their roles in the metabolism of nitrogenous compounds, and provides an interactive tool for visualizing these data. We further characterize several aminotransferases, lactamases, and regulators, which are of particular interest for metabolic engineering.