Biocatalytic kinetic resolution of <scp>d</scp>,<scp>l</scp>-pantolactone by using a novel recombinant <scp>d</scp>-lactonase
Qiu-Hua Zhang, Yi Fang, Wen-Fang Luo, Liu-Nv Huang
Abstract
host. The recombinant TSDL exhibited high hydrolysis activity and enantioselectivity toward d-pantolactone. The reaction conditions of the recombinant TSDL-catalyzed kinetic resolution of d,l-pantolactone was systematically investigated by whole cell biocatalysis. In addition, a preparative-scale reaction for bioproduction of d-pantoic acid was examined under optimized reaction conditions. This study presented an alternative enzymatic process for kinetic resolution of d,l-pantolactone.
Topics & Concepts
ChemistryKinetic resolutionBiocatalysisRecombinant DNABioproductionHydrolysisEnzymeStereochemistryResolution (logic)Enzyme kineticsCatalysisBiochemistryReaction mechanismEnantioselective synthesisActive siteArtificial intelligenceComputer scienceGeneMetabolism and Genetic DisordersNeurological diseases and metabolismEnzyme Catalysis and Immobilization