Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro
Sang Luo, Shuai Xiao, Yang Ai, Ben Wang, Yefu Wang
Abstract
BACKGROUND: -aged hUC-MSCs is needed. METHODS: and subcultured every 3 days up to P8, and their morphology, proliferative capacity, liver-specific marker expression, and liver function at the end of each passage were analyzed. The efficiency of the hepatogenic differentiation of hUC-MSCs driven by a functional hit 1 (FH1)-based strategy at different passages was also evaluated. RESULTS: -aged hUC-MSCs reduced significantly, and the mature hepatocyte functions, such as ALB secretion, glycogen synthesis, low-density lipoprotein (LDL) intake, and indocyanine green (ICG) uptake, also changed. CONCLUSIONS: -aged hUC-MSCs decreased dramatically in the late passage (P8). The current study provides valuable information can inform future research on liver disease.