Critical assessment of selenourea as an efficient small molecule fluorescence quenching probe to monitor protein dynamics
Subhrakant Jena, Kiran Devi Tulsiyan, Rudhi Ranjan Sahoo, Saiprakash Rout, Akshay Kumar Sahu, Himansu S. Biswal
Abstract
: 490-690 nm), were chosen to validate the proof of the concept. It was extended to tetramethylrhodamine (TMR)-5-maleimide labeled lysozyme protein, where we observed significant fluorescence quenching in the presence of SeU. The present work emphasizes that the high quenching efficiency with an ultrafast PET process, reduced orbital energy gap, and higher negative free energy change of the electron transfer reaction are the representative characteristics of selenourea or selenoamides to enable them as potential surrogates of thioamides or oxoamides quenching probes to monitor protein conformational changes and dynamics.
Topics & Concepts
ChemistryPhotoinduced electron transferPhotochemistryQuenching (fluorescence)Electron transferFluorescenceExcited stateMoleculeOrganic chemistryPhysicsQuantum mechanicsNuclear physicsPhotochemistry and Electron Transfer StudiesPorphyrin and Phthalocyanine ChemistryPhotosynthetic Processes and Mechanisms