Genome-wide mapping of <i>N</i><sup>4</sup>-methylcytosine at single-base resolution by APOBEC3A-mediated deamination sequencing
Jun Xiong, Ping Wang, Wen-Xuan Shao, Gaojie Li, Jiang-Hui Ding, Neng‐Bin Xie, Min Wang, Qing-Yun Cheng, Conghua Xie, Yu‐Qi Feng, Weimin Ci, Bi‐Feng Yuan
Abstract
, among which 564 sites were located in the CCGCGG motif. The average methylation levels in the CCGCGG motif and non-CCGCGG sequence were 70.0% and 22.8%, respectively. We envision that the 4mC-AMD-seq method will facilitate the investigation of 4mC functions, including the 4mC-involved R-M systems, in uncharacterized but potentially useful strains.
Topics & Concepts
DeaminationBase (topology)Resolution (logic)GeneticsChemistryBiologyComputer scienceMathematicsBiochemistryEnzymeProgramming languageMathematical analysisEpigenetics and DNA MethylationRNA modifications and cancerPolyamine Metabolism and Applications