Tumor dissociation of highly viable cell suspensions for single-cell omic analyses in mouse models of breast cancer
Laura Rodríguez de la Fuente, Andrew M. K. Law, David Gallego‐Ortega, Fátima Valdés‐Mora
Abstract
Cell preparation with a high rate of viable cells is required to obtain reliable single-cell transcriptomic and epigenomic data. This protocol describes a technique for digestion and single-cell isolation from mouse mammary tumors to achieve ∼90% of viable cells, which can be subsequently processed in a diverse array of high-throughput single-cell "omic platforms," both in an unbiased manner or after selection of a specific cell population. For complete details on the use and execution of this protocol, please refer to Valdes-Mora et al. (2021).
Topics & Concepts
Breast cancerCellDissociation (chemistry)Computational biologyCancerBiologyChemistryCancer researchBiochemistryGeneticsPhysical chemistrySingle-cell and spatial transcriptomicsCancer Cells and MetastasisCancer Genomics and Diagnostics