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Machine-Learning Model for Prediction of Cefepime Susceptibility in Escherichia coli from Whole-Genome Sequencing Data

Romney M. Humphries, Eugene Bragin, Julian Parkhill, Grace Morales, Jonathan E. Schmitz, Paul Rhodes

2023Journal of Clinical Microbiology27 citationsDOIOpen Access PDF

Abstract

The declining cost of performing bacterial whole-genome sequencing (WGS) coupled with the availability of large libraries of sequence data for well-characterized isolates have enabled the application of machine-learning (ML) methods to the development of nonlinear sequence-based predictive models. We tested the ML-based model developed by Next Gen Diagnostics for prediction of cefepime phenotypic susceptibility results in Escherichia coli .

Topics & Concepts

CefepimeBroth microdilutionBiologyEscherichia coliWhole genome sequencingClinical microbiologyMicrobiologyAntimicrobialGenomeMinimum inhibitory concentrationGeneticsCeftazidimeBacteriaGenePseudomonas aeruginosaBacterial Identification and Susceptibility TestingAntibiotic Resistance in BacteriaDiphtheria, Corynebacterium, and Tetanus
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