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Molecular Prevalence and Phylogenetic Characterization of <i>Enterocytozoon bieneusi</i> in Healthy Cattle

Tuğba Bilgin, Sadullah Uslu, Gupse Kübra Karademir, Mübeccel Okur, Gamze Yetişmiş, Alparslan Yıldırım

2020Turkish Journal of Parasitology21 citationsDOIOpen Access PDF

Abstract

Objective: in healthy cattle. Methods: positive isolates were sequenced for genotyping and phylogenetic analyzes. Obtained sequences were assembled with appropriative genetic software, then phylogenetic relationships were revealed. Results: . As a result of the sequence analyses, five distinct genotypes were determined. The most frequent genotype ERUSS1 and the other ERUSS2-4 genotypes were characterized as close to each other, which was reported for the first time in the world. Two isolates were determined in N genotype that was reported from cattle in Germany and were more different from the other genotypes. Phylogenetic analysis revealed that all the genotypes characterized in the study belonged to the genogroup 2. Conclusion: in cattle from Turkey were obtained with this study.

Topics & Concepts

GenotypeBiologyPhylogenetic treeEnterocytozoon bieneusiGenotypingNested polymerase chain reactionMolecular epidemiologygenomic DNARibosomal RNAFecesInternal transcribed spacerVeterinary medicineGenetic diversityGeneticsPolymerase chain reactionVirologyGeneMicrobiologyPopulationMedicineEnvironmental healthVeterinary medicine and infectious diseasesHelminth infection and controlParasitic Infections and Diagnostics
Molecular Prevalence and Phylogenetic Characterization of <i>Enterocytozoon bieneusi</i> in Healthy Cattle | Litcius