Typical Stress Granule Proteins Interact with the 3′ Untranslated Region of Enterovirus D68 To Inhibit Viral Replication
Jinyan Cheng, Shuai Gao, Cheng Zhu, Sihua Liu, Jinyu Li, Jun Kang, Zhiyun Wang, Tao Wang
Abstract
EV-D68 is a serious threat to human health, and there are currently no effective treatments or vaccines. SGs play an important role in cellular innate immunity as a target with antiviral effects. This manuscript describes the formation of SGs induced by EV-D68 early infection but inhibited during the late stage of infection. Moreover, TIA1, HUR, and G3BP1 can chelate a specific site of the 3' UTR of EV-D68 to inhibit viral replication, and this interaction is sequence and complex dependent. However, this inhibition can be antagonized by overexpression of the minireplicon. These findings increase our understanding of EV-D68 infection and may help identify new antiviral targets that can inhibit viral replication and limit the pathogenesis of EV-D68.