COVID-19 and fungal superinfection

Abstract

The Comment by Paul Verweij and colleagues1Verweij PE Gangneux J-P Bassetti M et al.Diagnosing COVID-19-associated pulmonary aspergillosis.Lancet Microbe. 2020; (published online May 8.)DOI:10.1016/S2666-5247(20)30027-6Summary Full Text Full Text PDF PubMed Scopus (153) Google Scholar on diagnosing COVID-19-associated pulmonary aspergillosis was insightful and much needed. We note some unanswered questions and review specific details of the studies cited. Zhang and colleagues2Zhang G Hu C Luo L et al.Clinical features and short-term outcomes of 221 patients with COVID-19 in Wuhan, China.J Clin Virol. 2020; 127104364Crossref PubMed Scopus (432) Google Scholar note that seven (3%) of 221 patients had co-infection with a fungus; however, there was no discussion regarding which fungi were isolated, nor any supporting evidence of invasive fungal infection (eg, by imaging or antigen testing) or treatment. Alanio and colleagues3Alanio A Dellière S Fodil S Bretagne S Mégarbane B High prevalence of putative invasive pulmonary aspergillosis in critically ill COVID-19 patients.MedRXiv. 2020; (published online April 24.) (preprint).DOI:10.1101/2020.04.21.20064915Google Scholar described nine (33%) of 27 patients admitted to the intensive care unit (ICU) as having invasive aspergillosis. However, only one patient, with concurrent candidaemia (Candida glabrata), received antifungal treatment (voriconazole). Supportive diagnostic criteria, including serum galactomannan and bronchoalveolar lavage galactomannan, were negative in all patients, and no deaths were attributed to invasive fungal infection. Koehler and colleagues4Koehler P Cornely OA Böttiger BW et al.COVID-19 associated pulmonary aspergillosis.Mycoses. 2020; (published online April 27.)DOI:10.1111/myc.13096Crossref Scopus (403) Google Scholar described invasive aspergillosis in five (26%) of 19 patients admitted to the ICU; three patients were positive for Aspergillus spp by PCR and galactomannan from a bronchoalveolar lavage sample, one patient grew Aspergillus spp on a tracheal aspirate but was negative for serum galactomannan, and the final patient had positive serum galactomannan with no growth on a tracheal aspirate. From our experience, if a patient does not respond to broad-spectrum antibacterials in the ICU we suggest the addition of an antifungal, liposomal amphotericin B. This recommendation was extrapolated from seasonal influenza findings in the absence of available COVID-19 epidemiology data.5Canning B Senanayake V Burns D Moran M Dedicoat M Post-influenza aspergillus ventriculitis.Clin Infect Pract. 2020; (published online April 5.)DOI:10.1016/j.clinpr.2020.100026Crossref Scopus (4) Google Scholar Of those patients admitted to the ICU, 24 (42%) of 57 received liposomal amphotericin B (median treatment of 6 days, range 1–16; Heard KL unpublished). No patients grew fungi from invasive samples, but 12 (50%) had fungi from superficial samples (rectal screening swabs, tracheal aspirates, or sputum); one of which had Aspergillus fumigatus in a sputum sample and 11 grew Candida spp from superficial swabs. 14 (58%) patients who started liposomal amphotericin B had a serum (1,3)-β-D-glucan antigen test: all were negative. The one patient who grew Aspergillus spp had a bronchoalveolar lavage 1 day before the sputum sample, which did not grow a fungus and had negative serum (1, 3)-β-D-glucan and galactomannan. Five (21%) patients developed acute kidney injury in the context of liposomal amphotericin B therapy. Given our findings among patients with COVID-19, which include a notable incidence of Candida spp, minimal definitive invasive fungal infection, and a potential drug toxicity, we would like to add to the research questions posed by Verweij and colleagues.1Verweij PE Gangneux J-P Bassetti M et al.Diagnosing COVID-19-associated pulmonary aspergillosis.Lancet Microbe. 2020; (published online May 8.)DOI:10.1016/S2666-5247(20)30027-6Summary Full Text Full Text PDF PubMed Scopus (153) Google Scholar We suggest COVID-19 fungal research should also explore invasive Candida spp as potential pathogens, the environmental factors (rapid changes to ICU capacity and infrastructure) that might increase the risk of COVID-19-associated pulmonary aspergillosis, and the potential harm of treating unproven invasive aspergillosis. LSPM has consulted for bioMerieux (2013–20), DNAelectronics (2015), Dairy Crest (2017–18), Pfizer (2018–20), and Umovis Lab (2020), received speaker fees from Profile Pharma (2018), received research grants from the National Institute for Health Research (2013–19), Leo Pharma (2016), and CW+ charity (2018–19), and received educational support from Eumedica (2016–17), outside of the submitted work. All other authors declare no competing interests. COVID-19 and fungal infection: the need for a strategic approachWe read with interest the Correspondence from Katie Heard and colleagues1 describing the absence of diagnostically confirmed COVID-19-associated pulmonary aspergillosis (CAPA) in patients who are critically ill and commend the authors for considering this potential superinfection. It is evident from their approach that empirical antifungal therapy does not seem to be beneficial. Five (21%) patients who received empirical antifungals had adverse effects and there were no cases of CAPA documented; therefore, this approach could increase patient morbidity without providing a survival benefit. Full-Text PDF Open Access

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