Reverse stable isotope labelling with Raman spectroscopy for microbial proteomics
Jiro Karlo, Ashish Kumar Dhillon, Soumik Siddhanta, Surya Pratap Singh
Abstract
Abstract Global proteome changes in microbes affect the survival and overall production of commercially relevant metabolites through different bioprocesses. The existing methods to monitor proteome level changes are destructive in nature. Stable isotope probing (SIP) coupled with Raman spectroscopy is a relatively new approach for proteome analysis. However, applying this approach for monitoring changes in a large culture volume is not cost‐effective. In this study, for the first time we are presenting a novel method of combining reverse SIP using 13 C‐glucose and Deuterium to monitor the proteome changes through Raman spectroscopy. The findings of the study revealed visible changes (blue shifts) in proteome related peaks that can be used for monitoring proteome dynamics, that is, synthesis of nascent amino acids and its turnover with time in a non‐destructive, cost‐effective, and label‐free manner.