Improving the catalytic performance of <i>Pichia pastoris</i> whole-cell biocatalysts by fermentation process
Denggang Wang, Meiqi Chen, Xin Zeng, Wenjie Li, Shuli Liang, Ying Lin
Abstract
whole-cell biocatalysts. This fermentation process was much simpler, more energy-saving, and greener than additional operating after collecting cells to improve the catalytic ability of whole-cell biocatalysts. Compared with the general fermentation process, the protein production capacity of cells did not decrease. Meanwhile, the activity of whole-cell biocatalysts was increased to 262%, which indicates that the permeability and space resistance were improved to relieve the transport-limitations. Furthermore, the induction time was reduced from 60 h to 36 h. The fermentation process offered significant advantages over traditional permeabilizing reagent treatment and ultrasonication treatment based on the high efficiency and simplicity.