Occurrence and Antimicrobial Susceptibility Profile of <i>Salmonella</i> spp. in Raw and Ready-To-Eat Foods and <i>Campylobacter</i> spp. in Retail Raw Chicken Meat in Transylvania, Romania
Emil Tîrziu, Gabriel Bărbălan, Adriana Morar, Viorel Herman, Romeo Teodor Cristina, Kálmán Imre
Abstract
The survey was undertaken to investigate the presence and antimicrobial susceptibility profile of Salmonella spp. in raw and ready-to-eat (RTE) foods, and Campylobacter spp. in the retail raw chicken meat collected in two counties of Transylvania, Romania. A total of 13.1% (51/388) of the examined food samples were found to be Salmonella positive, with a distribution of 14.7% (48/326) in the raw food (i.e., pork, chicken carcass, and shell egg) and 4.8% (3/62) in the RTE samples (i.e., sausages, but not ham and salami), respectively. These differences were statistically significant ( p = 0.034). The isolates were serotyped as Salmonella Infantis ( n = 19), Salmonella Typhimurium ( n = 11) Salmonella Rissen ( n = 8), Salmonella Derby ( n = 3), Salmonella Enteritidis ( n = 3), Salmonella Bredeney ( n = 2), Salmonella Brandenburg ( n = 1), Salmonella Gloucester ( n = 1), Salmonella Goldcoast ( n = 1), Salmonella Kottbus ( n = 1), and Salmonella Ruzizi ( n = 1). Campylobacter strains were present in 29.4% (10/34) of the investigated chicken samples, and the identified species were Campylobacter coli (70%) and C. jejuni (30%). From the 14 tested antimicrobials, the Salmonella isolates were resistant against azithromycin (88.2%), tetracycline (54.9%), sulfamethoxazole (54.9%), ciprofloxacin (45.1%), nalidixic acid (43.1%), ampicillin (35.3%), chloramphenicol (33.3%), tigecycline (25.5%), cefotaxime (13.7%), colistin (13.7%), trimethoprim (7.8%), and gentamicin (2%), resulting in the expression of 21 multidrug-resistant (MDR) profiles. Of 10 Campylobacter isolates, 80% were resistant to ciprofloxacin and nalidixic acid, 40% to tetracycline, and 10% to streptomycin and erythromycin, respectively. Our findings indicate that Romanian isolates of Salmonella spp. and Campylobacter spp., contaminating animal-origin foods, can exhibit MDR patterns, representing a public health risk.