Lipidomics reveal the cognitive improvement effects of <i>Acer truncatum Bunge</i> seed oil on hypoxic–ischemic encephalopathy rats
Xianyang Chen, Wangting Song, Yige Song, Hongli Cao, Xiao Xu, Shujia Li, Yanmin Fu, Teng Xue, Chunguang Ding, Feng Lin, Yuan Shi, Jiujun Li
Abstract
seed oil (ASO) reduced brain damage and improved cognitive ability in HIE rats. Using lipidomic strategies, we observed that HIE rats had decreased unsaturated fatty acids and increased lysophospholipids in the brain. However, after 30 days of ASO treatment, phospholipids, plasmalogens, and unsaturated fatty acids increased, while lysophospholipids and oxidized glycerophospholipids decreased in both serum and the brain. Enrichment analysis showed that ASO intake mainly affected sphingolipid metabolism, fat digestion and absorption, glycerolipid metabolism and glycerophospholipid metabolic pathways in serum and the brain. Cluster, correlation, and confirmatory factor analyses showed that cognitive improvement after ASO administration was attributed to increased essential phospholipids and ω3/6/9 fatty acids, coupled with decreased oxidized glycerophospholipids in HIE rats. Our findings indicate that ASO has the potential to be developed as an effective food supplement for ischemic hypoxic newborns.