Using the High-Entropy Approach to Obtain Multimetal Oxide Nanozymes: Library Synthesis, <i>In Silico</i> Structure–Activity, and Immunoassay Performance
Thüong Phan‐Xuan, Simon Schweidler, Steffen Hirte, Moritz Schüller, Ling Lin, Anurag Khandelwal, Kai Wang, Jan Schützke, Markus Reischl, Christian Kübel, Horst Hahn, Gianluca Bello, Johannes Kirchmair, Jasmin Aghassi‐Hagmann, Torsten Brezesinski, Ben Breitung, Lea Ann Dailey
Abstract
High Resolution Image Download MS PowerPoint Slide High-entropy nanomaterials exhibit exceptional mechanical, physical, and chemical properties, finding applications in many industries. Peroxidases are metalloenzymes that accelerate the decomposition of hydrogen peroxide. This study uses the high-entropy approach to generate multimetal oxide-based nanozymes with peroxidase-like activity and explores their application as sensors in ex vivo bioassays. A library of 81 materials was produced using a coprecipitation method for rapid synthesis of up to 100 variants in a single plate. The A and B sites of the magnetite structure, (AA‘)(BB’B‘‘) 2 O 4, were substituted with up to six different cations (Cu/Fe/Zn/Mg/Mn/Cr). Increasing the compositional complexity improved the catalytic performance; however, substitutions of single elements also caused drastic reductions in the peroxidase-like activity. A generalized linear model was developed describing the relationship between material composition and catalytic activity. Binary interactions between elements that acted synergistically or antagonistically were identified, and a single parameter, the mean interaction effect, was observed to correlate highly with catalytic activity, providing a valuable tool for the design of high-entropy-inspired nanozymes.