carba Nicotinamide Adenine Dinucleotide Phosphate: Robust Cofactor for Redox Biocatalysis
Ioannis Zachos, Manuel Döring, Georg Tafertshofer, Robert C. Simon, Volker Sieber
Abstract
Abstract Here we report a new robust nicotinamide dinucleotide phosphate cofactor analog (carba‐NADP + ) and its acceptance by many enzymes in the class of oxidoreductases. Replacing one ribose oxygen with a methylene group of the natural NADP + was found to enhance stability dramatically. Decomposition experiments at moderate and high temperatures with the cofactors showed a drastic increase in half‐life time at elevated temperatures since it significantly disfavors hydrolysis of the pyridinium‐N−glycoside bond. Overall, more than 27 different oxidoreductases were successfully tested, and a thorough analytical characterization and comparison is given. The cofactor carba‐NADP + opens up the field of redox‐biocatalysis under harsh conditions.