Purification and characterization of alginate extracted from Sargassum hemiphyllum and its antioxidant and wound healing efficacy
Durairaj Karthick Rajan, Lingwen Zhang, Hao Li, Jie Li, Xiaotang Di, Shubing Zhang
Abstract
Seaweed derived polymers have been extensively utilized in food and pharmaceutical applications. The natural polysaccharide alginate was extracted from Sargassum hemiphyllum and the physicochemical properties of sodium alginate were confirmed using a variety of analytical techniques. The FT-IR spectral peaks at 1617 cm −1 and 2922 cm −1 position confirmed the existence of carboxylate (O–C–O) and CH stretching functional groups. The XRD data revealed 2θ values of 13.71°, 21.80° and 40.31° and demonstrating that the extracted sodium alginate was pure and semi-crystalline in nature. A concentration of 8 mg/mL sodium alginate showed a higher antioxidant potential and their effectiveness was confirmed using FRAP (ferric reducing antioxidant power) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assays. Furthermore, the scratch assay results showed that the rate of wound closure was high on sodium alginate treatment. Overall, the sodium alginate did not show any toxicity on HUVEC cells and exhibited good biocompatibility . The ultimate purpose of this study is to extract the alginate using conventional chemical approach and to validate the cytotoxic impacts on HUVECs cells.