Single-cell RNA sequencing highlights intratumor heterogeneity and intercellular network featured in adamantinomatous craniopharyngioma
Yu Jiang, Jin‐Long Yang, Jin‐Long Yang, Ruichao Liang, Xin Zan, Rangrang Fan, Baoyin Shan, Hao Liu, Li Li, Yue Wang, Min Wu, Xin Qi, Hongxu Chen, Qingqing Ren, Zhiyong Liu, Yuelong Wang, Jing Zhang, Peizhi Zhou, Qiang Li, Meng Tian, Jinhao Yang, Jinhao Yang, C Wang, Xueying Li, Shu Jiang, Liangxue Zhou, Gao Zhang, Yaohui Chen, Jianguo Xu
Abstract
Despite improvements in microscopically neurosurgical techniques made in recent years, the prognosis of adamantinomatous craniopharyngioma (ACP) is still unsatisfactory. Little is known about cellular atlas and biological features of ACP. Here, we carried out integrative analysis of 44,038 single-cell transcriptome profiles to characterize the landscape of intratumoral heterogeneity and tumor microenvironment (TME) in ACP. Four major neoplastic cell states with distinctive expression signatures were defined, which further revealed the histopathological features and elucidated unknown cellular atlas of ACP. Pseudotime analyses suggested potential evolutionary trajectories between specific neoplastic cell states. Notably, a distinct oligodendrocyte lineage was identified in ACP, which was associated with immunological infiltration and neural damage. In addition, we described a tumor-centric regulatory network based on intercellular communication in TME. Together, our findings represent a unique resource for deciphering tumor heterogeneity of ACP, which will improve clinical diagnosis and treatment strategies.