Improved fluorescent <i>Listeria</i> spp. biosensors for analysis of antimicrobials by flow cytometry
Sebastian J. Reich, Jonas Stohr, Oliver Goldbeck, Bastian Fendrich, Peter Crauwels, Christian U. Riedel
Abstract
The global increase in antibiotic resistance of pathogenic microorganisms requires the identification and characterization of novel antimicrobials. Bacterial biosensors expressing fluorescent proteins such as pHluorin variants are suitable for high-throughput screenings. Here, we present Listeria spp. pH-sensitive biosensors with improved fluorescence for single-cell analysis of antimicrobials by flow cytometry.
Topics & Concepts
Flow cytometryListeriaListeria monocytogenesAntimicrobialMicrobiologyBiologyMolecular biologyBacteriaGeneticsInnovative Microfluidic and Catalytic Techniques InnovationListeria monocytogenes in Food SafetyBiosensors and Analytical Detection