Elucidating the solution structure of the monomolecular <i>BCL2</i> RNA G-quadruplex: a new robust NMR assignment approach
Zenghui Wang, Carla Ferreira Rodrigues, Simon Jurt, Alicia Domínguez‐Martín, Silke Johannsen, Roland K. O. Sigel
Abstract
proto-oncogene, one of the first natural monomolecular RNA G4 structures available to date. Comparative analysis with other RNA and DNA G4s reveals their notably compact and well-defined cores. Moreover, the sugar pucker geometries of the tetrad guanines are far less stringent than previously assumed, adeptly accommodating specific structural features. This contrasts with the canonical base pairing in RNA and DNA, in which the sugar pucker dictates the type of the double-helical structure. The strategy presented provides a direct path to uncovering G4 structural intricacies, advancing our grasp of their biological roles, and paving the way for RNA-targeted therapeutics.