Genetic control of violacein biosynthesis to enable a pigment-based whole-cell lead biosensor
Chang-ye Hui, Yan Guo, Lisa Liu, Nai-xing Zhang, Chao-xian Gao, Xueqin Yang, Juan Yi
Abstract
quantification of violacein extracted by butanol, which is significantly lower than reported fluorescent protein-based PbrR-regulated biosensors based on direct measurement of whole cell fluorescence. These results indicate that genetically controlled violacein biosynthesis can enable a sensitive, visual, and qualitative biosensor for monitoring the presence of bioavailable Pb(ii) in lead-contaminated water.
Topics & Concepts
BiosensorLead (geology)BiosynthesisPigmentChemistryComputational biologyCellBiochemistryCell biologyBiologyGenePaleontologyOrganic chemistryMicrobial Metabolism and ApplicationsMedicinal Plants and Neuroprotectionbioluminescence and chemiluminescence research