Effects of compound probiotics and aflatoxin-degradation enzyme on alleviating aflatoxin-induced cytotoxicity in chicken embryo primary intestinal epithelium, liver and kidney cells
Hongwei Guo, Juan Chang, Ping Wang, Qingqiang Yin, Chaoqi Liu, Xiaoxiang Xu, Xiaowei Dang, Xiaofei Hu, Quanliang Wang
Abstract
Abstract Aflatoxin B 1 (AFB 1 ) is one of the most dangerous mycotoxins for humans and animals. This study aimed to investigate the effects of compound probiotics (CP), CP supernatant (CPS), AFB 1 -degradation enzyme (ADE) on chicken embryo primary intestinal epithelium, liver and kidney cell viabilities, and to determine the functions of CP + ADE (CPADE) or CPS + ADE (CPSADE) for alleviating cytotoxicity induced by AFB 1 . The results showed that AFB 1 decreased cell viabilities in dose-dependent and time-dependent manners. The optimal AFB 1 concentrations and reactive time for establishing cell damage models were 200 µg/L AFB 1 and 12 h for intestinal epithelium cells, 40 µg/L and 12 h for liver and kidney cells. Cell viabilities reached 231.58% ( p < 0.05) for intestinal epithelium cells with CP addition, 105.29% and 115.84% ( p < 0.05) for kidney and liver cells with CPS additions. The further results showed that intestinal epithelium, liver and kidney cell viabilities were significantly decreased to 87.12%, 88.7% and 84.19% ( p < 0.05) when the cells were exposed to AFB 1 ; however, they were increased to 93.49% by CPADE addition, 102.33% and 94.71% by CPSADE additions ( p < 0.05). The relative mRNA abundances of IL-6, IL-8, TNF-α, iNOS, NF-κB, NOD1 (except liver cell) and TLR2 in three kinds of primary cells were significantly down-regulated by CPADE or CPSADE addition, compared with single AFB 1 group ( p < 0.05), indicating that CPADE or CPSADE addition could alleviate cell cytotoxicity and inflammation induced by AFB 1 exposure through suppressing the activations of NF-κB, iNOS, NOD1 and TLR2 pathways.