Concurrent stem- and lineage-affiliated chromatin programs precede hematopoietic lineage restriction
Fatemeh Safi, Parashar Dhapola, Sarah Warsi, Mikael N.E. Sommarin, Eva Erlandsson, Jonas Ungerbäck, Rebecca Warfvinge, Ewa Sitnicka, David Bryder, Charlotta Böiers, Ram Krishna Thakur, Göran Karlsson
Abstract
cells that concurrently display stem-like and lineage-affiliated chromatin signatures, pointing to a simultaneous gain of both lympho-myeloid and megakaryocyte-erythroid programs. Molecularly and functionally, these cells position between stem cells and committed progenitors and display multi-lineage capacity in vitro and in vivo but lack self-renewal activity. This integrative molecular analysis resolves the heterogeneity of cells along hematopoietic differentiation and permits investigation of chromatin-mediated transition between multipotency and lineage restriction.