Reconstitution of cargo-induced LC3 lipidation in mammalian selective autophagy
Chunmei Chang, Xiaoshan Shi, Liv Jensen, Adam L. Yokom, Dorotea Fracchiolla, Sascha Martens, James H. Hurley
Abstract
as a model cargo, the cargo receptors NDP52, TAX1BP1, and OPTN, and the autophagy core complexes. All three cargo receptors potently stimulated LC3 lipidation on GUVs. NDP52- and TAX1BP1-induced LC3 lipidation required all components, but not ULK1 kinase activity. However, OPTN bypassed the ULK1 requirement. Thus, cargo-dependent stimulation of LC3 lipidation is common to multiple autophagic cargo receptors, yet the details of core complex engagement vary between the different receptors.
Topics & Concepts
AutophagyLipid-anchored proteinCell biologyReceptorBiologyGeneticsApoptosisAutophagy in Disease and TherapyPancreatic function and diabetesCalcium signaling and nucleotide metabolism