Fast and Highly Efficient Affinity Enrichment of Azide-A-DSBSO Cross-Linked Peptides
Manuel Matzinger, Wolfgang Kandioller, Philipp Doppler, Elke H. Heiß, Karl Mechtler
Abstract
ribosomes in a background of 100 μg tryptic HEK peptides, indicating a high enrichment sensitivity. In contrast to conventional enrichment techniques, like SEC, the time needed for preparation and MS measurement is significantly reduced. This robust, fast, and selective enrichment method for azide-tagged linkers will contribute to mapping protein-protein interactions, investigating protein architectures in more depth, and helping to understand complex biological processes.
Topics & Concepts
StreptavidinChemistryAzideRibosomeMass spectrometryPeptideChromatographyBiotinBiochemistryCombinatorial chemistryRNAGeneOrganic chemistryAdvanced Proteomics Techniques and ApplicationsBiotin and Related StudiesMass Spectrometry Techniques and Applications