Stalling chromophore synthesis of the fluorescent protein Venus reveals the molecular basis of the final oxidation step
Husam Sabah Auhim, Bella L. Grigorenko, Tessa Harris, Ozan Aksakal, Igor V. Polyakov, Colin Berry, Gabriel dos Passos Gomes, Igor V. Alabugin, P.J. Rizkallah, Alexander V. Nemukhin, D. Dafydd Jones
Abstract
Fluorescent proteins (FPs) have revolutionised the life sciences, but the chromophore maturation mechanism is still not fully understood. Here we photochemically trap maturation at a crucial stage and structurally characterise the intermediate.
Topics & Concepts
ChromophoreFluorescenceFluorescent proteinChemistryVenusBiophysicsPhotochemistryGreen fluorescent proteinBiochemistryBiologyAstrobiologyPhysicsOpticsGeneAdvanced Fluorescence Microscopy TechniquesPhotoreceptor and optogenetics researchPhotosynthetic Processes and Mechanisms