Real-time visualization of butyrylcholinesterase activity using a highly selective and sensitive chemiluminescent probe
Alperen Acari, Toghrul Almammadov, Musa Dirak, Goktug Gulsoy, Safacan Kölemen
Abstract
imaging ability of BCC was demonstrated in healthy and tumor-bearing mice models. BCC enabled us to visualize the BChE activity in different regions of the body. Furthermore, it was successfully employed to monitor tumors derived from neuroblastoma cells with a very high signal to noise ratio. Thus, BCC appears as a highly promising chemiluminescent probe, which can be used to further understand the contribution of BChE to regular cellular processes and the formation of diseased states.
Topics & Concepts
ButyrylcholinesteraseChemiluminescenceIn vivoEndogenyBiophysicsChemistryCholinesteraseNanotechnologyMaterials scienceBiologyEnzymeBiochemistryAcetylcholinesteraseAchéPharmacologyChromatographyBiotechnologyCholinesterase and Neurodegenerative DiseasesNicotinic Acetylcholine Receptors StudyMedicinal Plants and Neuroprotection