Protein S-nitrosylation during induced autophagy in Triticum aestivum
Anastasia Mazina, Natalia Gazizova, Amina G. Daminova, F. V. Minibayeva
Abstract
Autophagy is a highly conservative catabolic process involved in the development of tissues and stress response of cells. Among the most important components of the induction and maintenance of autophagy, the posttranslational modifications of proteins act as molecular triggers of signaling cascades. S-nitrosylation of proteins, which is the covalent attachment of nitric oxide group to cysteine thiol in a protein resulting in the formation of S-nitrosothiol, is a mechanism of NO-mediated signal transduction in many processes, including autophagy. This work is devoted to the analysis of protein S-nitrosylation during the induction of autophagy in the cells of wheat roots. Hypothetical S-nitrosylation sites of autophagic proteins were identified in wheat. Nitrosylated proteins were visualized in the roots where autophagy was induced by nitric oxide donor KNO2 and mitochondrial inhibitor antimycin A.