Introduction of Two Prolines and Removal of the Polybasic Cleavage Site Lead to Higher Efficacy of a Recombinant Spike-Based SARS-CoV-2 Vaccine in the Mouse Model
Fatima Amanat, Shirin Strohmeier, Raveen Rathnasinghe, Michael Schotsaert, Lynda Coughlan, Adolfo García‐Sastre, Florian Krammer
Abstract
A vaccine for SARS-CoV-2 is urgently needed. A better understanding of antigen design and attributes that vaccine candidates need to have to induce protective immunity is of high importance. The data presented here validate the choice of antigens that contain the PP mutations and suggest that deletion of the polybasic cleavage site may lead to a further-optimized design.
Topics & Concepts
Cleavage (geology)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)AntigenRecombinant DNALead (geology)Computational biologyCoronavirus disease 2019 (COVID-19)2019-20 coronavirus outbreakImmunityVirologyImmunologyBiologyMedicineImmune systemGeneticsDiseaseInfectious disease (medical specialty)PathologyPaleontologyGeneFracture (geology)OutbreakSARS-CoV-2 and COVID-19 ResearchAnimal Virus Infections StudiesViral gastroenteritis research and epidemiology