A biocomplex to repair experimental critical size defects associated with photobiomodulation therapy
Daniela Vieira Buchaim, Jesus Carlos Andreo, Karina Torres Pomini, Benedito Barraviera, Rui Seabra Ferreira Júnior, Marco Antonio Hungaro Duarte, Murilo Priori Alcalde, Carlos Henrique Bertoni Reis, Daniel de Bortoli Teixeira, Cleuber Rodrigo de Souza Bueno, Cláudia Rucco Penteado Detregiachi, Adriano Cressoni Araujo, Rogério Leone Buchaim
Abstract
Background: The association of scaffolds to repair extensive bone defects can contribute to their evolution and morphophysiological recomposition. The incorporation of particulate biomaterials into three-dimensional fibrin bioproducts together with photobiomodulation therapy (PBM) has potential and can improve regenerative medicine procedures. The objective of this experiment was to evaluate the effects of PBM therapy on critical size defects filled with xenogenic bone substitute associated with fibrin biopolymer. Methods: , energy/point 0.72 J, total energy 2.88 J. Fourteen and 42 days after the surgery, animals were euthanatized and subjected to microtomography, qualitative and quantitative histological analysis. Results: = 0.07093, respectively). Intense vascular proliferation and bone deposition around the biomaterial particles were observed in the animals of the groups in which biocomplex was applied (XS and XS-PBM). Conclusion: PBM therapy allowed an improvement in the formation of new bone, with a more organized deposition of collagen fibers in the defect area. Biocomplex favored the insertion and permanence of the particulate material in bone defects, creating a favorable microenvironment for accelerate repair process.