Depletion of METTL3 alters cellular and extracellular levels of miRNAs containing m6A consensus sequences
Jessica J. Abner, Jeffrey L. Franklin, Margaret A. Clement, Scott A. Hinger, Ryan M. Allen, Xiao Liu, Stefanie Kellner, Junzhou Wu, John Karijolich, Qi Liu, Kasey C. Vickers, Peter C. Dedon, Alissa M. Weaver, Robert J. Coffey, James G. Patton
Abstract
A modification. Functional testing of EVs prepared from cells expressing shRNAs against METTL3 showed that they were less capable of conferring colony growth in 3D to wild-type KRAS cells and were also largely incapable of conferring the spread of cetuximab resistance. Our data support a role for METTL3 modification on cellular miRNA levels and export of specific miRNAs.
Topics & Concepts
KRASmicroRNAGene knockdownExtracellularBiologyCell biologyExtracellular vesiclesMutantMicrovesiclesExtracellular vesicleWild typeCancer researchColorectal cancerCell cultureGeneticsGeneCancerRNA modifications and cancerRNA Research and SplicingCancer-related molecular mechanisms research