CRISPR-Cas9 screen identifies oxidative phosphorylation as essential for cancer cell survival at low extracellular pH
Johanna Michl, Yunyi Wang, Stefania Monterisi, Wiktoria Błaszczak, Ryan Beveridge, Esther Bridges, Jana Koth, Walter F. Bodmer, Pawel Swietach
Abstract
cells grew considerably slower than their wild-type controls, but growth could be stimulated with systemic bicarbonate therapy that lessens the tumoral acid stress. These findings raise the possibility of therapeutically targeting mitochondrial metabolism in combination with acid stress as a cancer treatment option.
Topics & Concepts
CRISPROxidative phosphorylationExtracellularPhosphorylationCell survivalCell biologyCellExtracellular vesiclesOxidative stressChemistryBiologyCancer researchBiochemistryGeneApoptosisEnzyme function and inhibitionElectrochemical sensors and biosensorsATP Synthase and ATPases Research