Extracellular vesicles originating from autophagy mediate an antibody-resistant spread of classical swine fever virus in cell culture
Tao Wang, Liang Zhang, Wulong Liang, Shanchuan Liu, Wen Deng, Yangruiyu Liu, Yaru Liu, Mengzhao Song, Kangkang Guo, Yanming Zhang
Abstract
: 3-MA: 3-methyladenine; CCK-8: Cell Counting Kit-8; CSF: classical swine fever; CQ: chloroquine; CSFV: classical swine fever virus; DAPI, 4-,6-diamidino-2-phenylindole; EVs: extracellular vesicles; hpi: h post infection; IEM: immunoelectron microscopy; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MOI: multiplicity of infection; MVs: microvesicles; ND50: half neutralizing dose; PCR: polymerase chain reaction; PBS: phosphate-buffered saline; SEC: size-exclusion chromatography; siRNA: small interfering RNA; TEM: transmission electron microscopy.
Topics & Concepts
BiologyAutophagyClassical swine feverVirusVirologyExtracellularCell biologyAntibodyViral replicationApoptosisImmunologyBiochemistryAnimal Disease Management and EpidemiologyExtracellular vesicles in diseaseViral Infections and Vectors