Characterization of a GH8 β-1,4-Glucanase from <i>Bacillus subtilis</i> B111 and Its Saccharification Potential for Agricultural Straws
Zhen Huang, Guorong Ni, Xiaoyan Zhao, Fei Wang, Mingren Qu
Abstract
values of celA1805 were 1.78 mg/ml and 50.09 μmol/min/mg. When incubated with cellooligosaccharides ranging from cellotriose to cellopentose, celA1805 mainly hydrolyzed cellotetrose (G4) and cellopentose (G5) to cellose (G2) and cellotriose (G3), but hardly hydrolyzed cellotriose. The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/ml, respectively. The results obtained in this study suggest potential applications of celA1805 in biomass saccharification.
Topics & Concepts
Bacillus subtilisHydrolysisGlucanaseStrawChemistryGlycosylFood scienceSubstrate (aquarium)BacillalesHydrolaseEnzymeBiochemistryBacteriaBiologyGeneticsInorganic chemistryEcologyBiofuel production and bioconversionEnzyme Production and CharacterizationEnzyme Catalysis and Immobilization