The yeast platform engineered for synthetic gRNA-landing pads enables multiple gene integrations by a single gRNA/Cas9 system
Sihyun Baek, Joseph Christian Utomo, Ji Young Lee, Kunal Dalal, Yeo Joon Yoon, Dae‐Kyun Ro
Topics & Concepts
CRISPRBiologyCas9MultiplexGuide RNAGeneComputational biologyGenome editingSaccharomyces cerevisiaeSynthetic biologyYeastGeneticsGreen fluorescent proteinPlant tissue culture and regenerationBotanical Research and ApplicationsPlant biochemistry and biosynthesis