A peptide-based subunit candidate vaccine against SARS-CoV-2 delivered by biodegradable mesoporous silica nanoparticles induced high humoral and cellular immunity in mice
Lei Qiao, Minmin Chen, Suyan Li, Jinxia Hu, Chaoju Gong, Zhuoqi Zhang, Xichuan Cao
Abstract
, could efficiently deliver epitope peptides into the cytoplasm of RAW264.7 cells. Strong Th1-biased humoral and cellular immunity were induced by B/T@BMSNs in mice and all the 10 selected epitopes were identified as effective antigen epitopes, which could induce robust peptide-specific immune response. The elicited functional antibody could bind to the recombinant S protein and block the binding of the S protein to the ACE-2 receptor. These results demonstrate the potential of a nanoparticles vaccine platform based on BMSNs to rapidly develop peptide-based subunit vaccine candidates against SARS-CoV-2.
Topics & Concepts
EpitopePeptidePeptide vaccineProtein subunitImmune systemAntigenHumoral immunityChemistryAntibodyVirologyImmunityBiologyBiochemistryImmunologyGeneImmunotherapy and Immune Responsesvaccines and immunoinformatics approachesSARS-CoV-2 and COVID-19 Research