Planning and Analyzing a Low-Biomass Microbiome Study: A Data Analysis Perspective
George I. Austin, Tal Korem
Abstract
As investigations of low-biomass microbial communities have become more common, so too has the recognition of major challenges affecting these analyses. These challenges have been shown to compromise biological conclusions and have contributed to several controversies. Here, we review some of the most common and influential challenges in low-biomass microbiome research. We highlight key approaches to alleviate these potential pitfalls, combining experimental planning strategies and data analysis methods.
Topics & Concepts
MicrobiomePerspective (graphical)CompromiseBiomass (ecology)Data scienceKey (lock)Risk analysis (engineering)Environmental resource managementBiologyComputer scienceEcologyBioinformaticsMedicineSociologyEnvironmental scienceArtificial intelligenceSocial scienceMicrobial Metabolic Engineering and BioproductionGut microbiota and healthBioinformatics and Genomic Networks