Electrospinning Live Cells Using Gelatin and Pullulan
Nasim Nosoudi, Anson Jacob Oommen, Savannah Stultz, Micah Jordan, Seba Aldabel, Chandra Hohne, James Mosser, Bailey Archacki, Alliah Turner, Paul Turner
Abstract
Electrospinning is a scaffold production method that utilizes electric force to draw a polymer solution into nanometer-sized fibers. By optimizing the polymer and electrospinning parameters, a scaffold is created with the desired thickness, alignment, and pore size. Traditionally, cells and biological constitutes are implanted into the matrix of the three-dimensional scaffold following electrospinning. Our design simultaneously introduces cells into the scaffold during the electrospinning process at 8 kV. In this study, we achieved 90% viability of adipose tissue-derived stem cells through electrospinning.