Green coffee VS dietary supplements: A comparative analysis of bioactive compounds and antioxidant activity
Justyna Brzezicha, Daria Błażejewicz, Joanna Brzezińska-Rojek, Małgorzata Grembecka
Abstract
The aim of this work was to assess and compare both the antioxidant potential and content of the selected biologically active substances in green coffee samples and dietary supplements based on green coffee extracts. The newly developed method using HPLC coupled with Corona detector (HPLC-CAD) allowed the analysis of chlorogenic acid, caffeine, caffeic acid, coumaric acid, and ferulic acid in a single run. The method was validated and characterized by a wide concentration range (5-75 μg/mL), sensitivity, accuracy (92.7-112%), repeatability (RSD <5.32%), and precision (1.80-4.04%). The assessment of antioxidant potential was done by the spectrophotometric Folin-Ciocalteu method. Green coffee samples and extract characterized by comparable or, in the case of some samples, even higher antioxidant properties than dietary supplements. There was found a great variety of CGA levels in dietary supplements (0.33-329 mg/g) compared to the green coffee samples (32.7 mg/g - 47.6 mg/g). The highest caffeine content (156 mg/tablet) was determined in one dietary supplement, while green coffee samples contained its lower levels (20.9 mg/g). The quality of some supplements is not satisfactory as their composition does not comply with the manufacturer's declaration. There were found discrepancies between the value determined and declared by the producer (<243%).