An effective biocompatible fluorescent probe for bisulfite detection in aqueous solution, living cells, and mice
Ruqiao Zhou, Guiling Cui, Yuefu Hu, Qingrong Qi, Wencai Huang, Li Yang
Abstract
, and undergoes 1,4-addition reactions at the C-4 atom of the ethylene group. The reaction can be completed in 30 s in a PBS buffer solution and displays high sensitivity (limit of detection is 80 nM) for bisulfites. Test paper experiments show that the probe can be used for bisulfite detection in aqueous solutions. In addition, Hcy-Mo exhibits excellent cell permeability and low cytotoxicity for the successful detection of bisulfites in living MDA-MB-231 cells and in living mice, implying that this probe would be of great benefit to biological researchers for investigating the detailed biological and pharmacological functions of bisulfites in biological systems.
Topics & Concepts
BisulfiteSulfur dioxideAqueous solutionBiocompatible materialChemistryFluorescencePollutantSulfurAqueous mediumHuman healthEnvironmental chemistryNanotechnologyInorganic chemistryBiochemistryOrganic chemistryMaterials scienceBiomedical engineeringQuantum mechanicsPhysicsDNA methylationMedicineGene expressionEnvironmental healthGeneSulfur Compounds in BiologyPer- and polyfluoroalkyl substances researchNanoplatforms for cancer theranostics