GPA peptide inhibits NLRP3 inflammasome activation to ameliorate colitis through AMPK pathway
Zhao Deng, Jiangjin Ni, Xiaoyu Wu, Hongkui Wei, Jian Peng
Abstract
mice. In macrophage, GPA suppresses the assembly of NLRP3 inflammasome and GSDMD cleavage. Furthermore, GPA maintains mitochondrial homeostasis through inhibiting ROS, mtDNA and NLRP3 mitochondrial localization, with other signals related to NLRP3 inflammasome unaffected. Furthermore, the inhibitory effects of GPA on reactive oxygen species (ROS) are found to be achieved by increasing AMPK phosphorylation. Our results suggest that GPA inhibits NLRP3 inflammasome activation through increasing AMPK phosphorylation to suppress ROS, and can be applied in the prevention of colitis through targeting NLRP3.
Topics & Concepts
InflammasomeColitisPyroptosisAMPKReactive oxygen speciesChemistryPhosphorylationInflammatory bowel diseaseCell biologyInflammationUlcerative colitisProinflammatory cytokineImmunologyProtein kinase AMedicineBiochemistryBiologyDiseaseInternal medicineInflammasome and immune disordersImmune cells in cancerAdenosine and Purinergic Signaling